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Extraction method of 1- Deoxynojiri mycin

    In recent years, the research on alkaloid components is increasingly in-depth at home and abroad, and there are many literatures on its extraction technology.
    The main methods include frying, dipping, percolating, ultrasonic and reflux methods [64]. Among them, the frying method is heated for a long time and the temperature
    High, alkaloid easily destroyed; The process of impregnation and percolation is long, the amount of solvent is large, and the processing process of extraction liquid is complicated.
    The extraction rate of alkaloid was low. However, the study of mulberry branch is mainly seen in pharmacology, and the extraction, separation and purification process of its total alkaloids
    However, few reports have been reported. Li Yuliang et al. [65] from Chang ‘an University studied the extraction and separation of 1-deoxynojiri mycin from mulberry leaves
    The effects of extraction agent, extraction method, solid-liquid ratio, extraction times, time and other factors on the extraction rate were discussed
    Ring.
    Macroporous resin adsorption method
    Now domestic and foreign scholars use macroporous adsorption resin to purify alkaloids. Macroporous adsorption resin is a class of ion – free
    Group network structure polymer adsorbent, with strong adsorption, easy desorption, good mechanical strength, reversible
    Multiple use and low fluid resistance, etc. [66]. At present, this technology is used in the separation and purification of the effective components and parts of traditional Chinese medicine
    More and more attention has been paid to the extraction and refining of formula, chemical composition analysis of traditional Chinese medicine, and new drug development of traditional Chinese medicine [67]. but
    Yes, the application of macroporous adsorbent resin in the separation and purification of the effective components of Chinese herbal medicine is still lack of systematic research
    The parts and parameters are not exact. The research on the whole process optimization and the establishment of internal quality control index has not been reported
    The way.
    High performance countercurrent chromatography
    High-speed counter-current chromatography (HSCCC) is a liquid

    • Liquid distribution separation technology, HSCCC separation effect is good, less solvent dosage, wide application range, does not need solid support agent (load
      Body), there is no irreversible adsorption of separated components, avoiding sample loss and denaturation, its solvent system selection
      It can be done on a wider scale. So far, high speed countercurrent chromatography has been used to study amino acids, purines,
      Pyridine, indole plant hormones, antibiotics, acids, low peptide mixtures and flavonoids, alkaloids, anthraquinones in Chinese herbs
      The separation of various active ingredients such as derivatives has achieved good results [68-70].
      Ultrasonic extraction technique
      Ultrasonic processing is a kind of technology which is assisted by external field processing. It is a high frequency vibration emitted by ultrasonic generator
      The wave signal is converted by the transducer into high-frequency mechanical vibration and propagated to the solvent, that is, the strength generated by ultrasonic cavitation
      Shear forces can rupture plant cell walls, making it easier for cells to release contents, while perturbation effects promote solvent entry into extraction
      The substance cell accelerates the entry of the component into the solvent. It can shorten the extraction time and improve the extraction rate without destroying the structure of the extract.
      The selectivity of ultrasonic extraction is mainly realized through the selectivity of solvent.
      Studies have reported on glycosides, flavonoids, anthraquinones, natural flavors and vegetable oils [71].
      Supercritical fluid extraction (SFE) Supercritical fluid Extraction (SFE)
      Supercritical fluid extraction technology is a new chemical separation technology developed in recent years, which is known as the 21st century
      Solvent extraction technology. The supercritical fluid is the fluid above the critical temperature (tc) and the critical pressure (pc)
      The dual characteristics of a liquid, which has the same high diffusion coefficient and low viscosity as a gas, and the same density as a liquid,
      It has good solubility to substances and can be used as solvent for extraction and separation. It can be used as a solvent for supercritical fluid extraction
      Many, such as CO2, propane, ethane, ethylene, methanol, water, etc

    Of course, in addition to the above methods, there are flocculation clarifying method, membrane separation technology, semi-bionic extraction method, and on
    Which method is most suitable for extracting DNJ from mulberry leaves remains to be tested. From the literature analysis, the DNJ
    There are not many researches on extraction and purification, low extraction rate is common, and there is no complete extraction and purification process. period
    Hope to find out the best extraction process and can deal with a large number of total alkaloid separation method and simple, fast, solid
    So as to lay the foundation for the industrial production and quality control of mulberry leaf alkaloids
    To facilitate its food, medicine and other aspects of full development.

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