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Biological preparation of chitosan oligosaccharides

    Chito-oligosaccharide, also known as chito-oligosaccharide, scientific name 13-1, 4-oligo-glucosamine, is a brand new product obtained from chitosan by special biotechnology treatment. It is a low molecular weight product with good water solubility, large function and high biological activity. It has many unique functions, such as high solubility and easy absorption by organisms, which chitosan does not have, so it should be used in many industries. 1. Chitosan oligosaccharide is known as the sixth essential vital factor for human body, which has the functions of three regulation (immune regulation, PH regulation and hormone regulation), three lowering (lowering blood lipids, blood sugar and blood pressure), three discharging (discharging cholesterol, discharging heavy metal ions and discharging toxins) and three inhibiting (inhibiting cancer cells, cancer cell metastasis and inhibiting carcinotoxins). It is also an activating factor of intestinal probiotics such as bifidobacteria. Therefore, chitosan oligosaccharides are widely used in the manufacture of medical and health food. 2. Chitosan oligosaccharide can accelerate the metabolism and regeneration of epidermal cells and has excellent moisturizing and hygroscopic function, which can effectively prevent skin roughness and aging. This shell oligosaccharide also has the function of bacteriostasis, anti-skin diseases and absorption of ultraviolet line, especially for acne. The addition of chitosan oligosaccharides in hair care products can keep the film-forming permeability of the hair surface, wet and easy to comb, and can resist static electricity, dust, itching and dandruff. Chitosan oligosaccharides are widely used in cosmetics because of their good chemical stability, good compound performance with surfactant surface activity and increased transdermal absorption. 3. Chitosan oligosaccharide in other industries has remarkable effect on inhibiting spoilage bacteria, and it is a natural food preservative with excellent performance. Chitosan oligosaccharides are also widely used in agriculture because of their effectiveness in increasing fruit and vegetable yields, controlling pests and diseases, and proliferating beneficial bacteria in soil and biofilm fertilizers.

    The purpose of the invention is to provide a new method for the production of narrow molecular weight distributed chitosan oligosaccharides, obtain narrow molecular weight distributed chitosan oligosaccharides by enzymolysis from plants containing polysaccharides, create a new way of production and broaden the application field of narrow molecular weight distributed chitosan oligosaccharides. In order to achieve the above purpose, the invention adopts the following technical scheme, the invention takes plants as raw materials, and the production process mainly includes pretreatment, enzymatic hydrolysis, inactivation, separation and screening steps. The pretreatment is to remove the sundries of the raw materials, clean them, dry them and then pulverize them into small particles; Enzymatic hydrolysis is to add powder and water to the reaction tank, stir, heat, and then add enzymes into the enzymatic hydrolysis reaction; Inactivation is when the enzymatic reaction reaches the requirements, the rapid heating, killing the activity of the enzyme, the termination of the enzymatic reaction; Solid-liquid separation is to centrifuge the material after enzymolysis to remove the larger particles, and then through pressure filtration, fine filtration to get clear liquid; Screening was conducted by ultrafiltration and reverse osmosis on the clear liquid with membrane to obtain narrow molecular weight distribution of chitosan oligosaccharide concentrate.

    The invention opens up a new way to produce chitosan oligosaccharides with narrow molecular weight distribution, the key of which lies in the control of enzymatic hydrolysis and inactivation. The selection of the process route is reasonable, with plants as raw materials, raw materials are easy to solve, the price is low, the production process is simple, easy to control, the production of narrow molecular weight distribution of chitosan oligosaccharide products have biological activity, it can induce plant synthesis, accumulate plant protection factor, antitoxin, and expand the application field of narrow molecular weight distribution of chitosan oligosaccharide, a new breakthrough in the application of biotechnology.

    The method takes the plants containing polysaccharides as raw materials, preprocesses the collected raw materials, cleans the sundries, and then air dries, pulverizes and sieving the powder. The powder can be directly supplied for enzymatic hydrolysis, or packaged and refrigerated for reserve. Add the powder and water into the reaction tank, stir and adjust the ra value to 3. 5. 5, heat to 30 55t:, add an appropriate amount of enzyme, and perform the enzymolysis reaction at a constant temperature. The heating temperature and the amount of enzyme will directly affect the enzymolysis reaction speed, and the heating temperature and the amount of enzyme will be directly proportional to the reaction speed. Rapid heating up to 8(TC above inactivation, inactivation of enzyme activity after cooling, enzymolysis reaction is used to degrade polysaccharide, enzyme activity exists, the reaction continues, the polysaccharide is reduced to narrow molecular weight distribution of chitosan oligosaccharides, and then degraded into monosaccharides, the reaction material is a mixture of a variety of sugars, our need is narrow molecular weight distribution of chitosan oligosaccharides, When the reaction is carried out to narrow molecular weight distribution of chitosan oligosaccharides most, polysaccharide, monosaccharides less, the requirements will be met, rapidly heating up the activity of killing enzyme, the termination of enzymatic hydrolysis reaction. After the enzymolysis of the material for solid-liquid separation, can be advanced coarse filter, remove the slag, and then remove the large particles on the centrifuge, through the plate frame pressure filtration, fine filtration, microhole filtration and other removal of small particles, to get clear liquid. Molecular screening was carried out on the clear liquid. Firstly, the membrane with 10000 molecular weight was selected for ultrafiltration, and the large molecular weight liquid was discarded and the permeable liquid was obtained. Then, the membrane with 500 molecular weight was selected and concentrated under certain pressure, and the small molecular liquid was discarded to obtain 500 10000 molecular weight concentrated liquid. If the production of water products, can be sterilized packaging as finished products; In the case of powdered products, the concentrate can be freeze-dried, crushed and screened for packaging into a finished product.

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