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Application of potassium 4-methoxy-salicylate

    Potassium 4-methoxy-salicylate (potassium 4-methoxy-salicylate) has a similar structure to salicylic acid. It is a new kind of medicinal whitening ingredient, which is used in cosmetics and plays a whitening role. It is an effective ingredient in cosmetics. Potassium 4-methoxy-salicylate can coordinate keratin metabolism, correct epidermal abnormal hyperplasia, promote the discharge of melanin, and achieve smooth and whitening skin; It can also block the source of melanine melanin generation and improve the self-healing power. It can improve the whitening effect when used with various types of whitening products and has a good development prospect.

    1. Inhibition of melanin production

    Mouse melanoma cells (B16) were cultured with and without potassium 4-methoxy-salicylate for 3 days, and the number of cells and the amount of melanin in a certain number of cells were measured. Compared with that without potassium 4-methoxy-salicylate, the number of cells and the ratio of melanin amount were obtained, and the effect of potassium 4-methoxy-salicylate on melanin production was analyzed in this way.

    1. Tyrosinase activity hindrance

    For the evaluation of tyrosinase activity in non-cellular systems, L DOPA was used to measure the amount of DOPA produced by metabolism with 475Mn absorbance test. The inhibition rate of tyrosinase activity of potassium 4-methoxy-salicylate was determined by adding potassium 4-methoxy-salicylate solution and solvent to the reaction system, and the mixture was kept at room temperature for 90 seconds to obtain the ratio of test values when adding potassium 4-methoxy-salicylate solution compared with the solvent group.

    1. It has inhibitory effect on the differentiation of keratinocytes caused by ultraviolet light

    After the 3d skin model was irradiated with ultraviolet light, 4-methoxy-salicylate potassium solution was added to the surface of the skin model immediately. After 30 minutes of culture, 4-methoxy-salicylate potassium solution was removed and continued to be cultured for 1 day. Instead of 4-methoxy-potassium salicylate solution, control with a solvent. The skin samples were sampled after culture, and the expression level of mDRin mRNA was quantified by real-time R-TPCR (reverse transcription polymerase chain reaction).

    1. Defense effect of pigmentation on human body

    Twelve healthy adult male volunteers were exposed to an artificial ultraviolet light source (UVB and UV) equivalent to IMED once a day for 3 consecutive days at 4 places on the abdomen. Four kinds of creams were used as test samples, including 1%, 3% and 5% 4-methoxy-salicylate potassium cream and matrix cream. They were applied 3 times a day for 7 consecutive days from the beginning of uv irradiation. To evaluate the degree of pigmentation, color tester was used to measure the skin color (L* value) of the sample before and after the application for three times, and the average value was obtained to obtain the change amount (△L*) before and after the irradiation. Then, different concentrations of 4-methoxy-potassium salicylate cream and matrix cream were compared.

    The preparation of

    (2) Add 150 grams of 4-methoxy-salicylic acid and 450 grams of deionized water into a 1000mL three-mouth bottle, heat it to 80℃ and stir for 30 minutes, then filter it while it is hot and dry to get 120 grams of white crystal.

    (3) In a 1000mL three-mouth bottle, add 600 grams of deionized water and 49.3 grams (0.357mol) of potassium carbonate, stir and dissolve, heat up to 50℃, and then slowly add 120 grams (0.714mol) of 4-methoxy-salicylic acid. During the feeding process, a large amount of carbon dioxide gas is released. After feeding, dissolve, After stirring for 0.5 hours, the solvent was recovered, and the temperature was reduced to 0 ~ 5℃ for crystallization. After filtration and drying, 110 grams of quasi-white crystal was obtained.

    (4) In a 1000ml three-mouth bottle, add 550 grams of 50% ethanol aqueous solution, 110 grams of crude 4-methoxy-salicylate potassium, heat to dissolve, add 10 grams of activated carbon, reflux for 1 hour, while hot extraction and filtration, cooling filtrate to 0℃, holding at 0 ~ 5℃ for 3 hours, extraction and vacuum drying, 90 grams of white crystal, the total yield is 60%.

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